Distinct expression and function of three ammonium transporter genes (OsAMT1;1 - 1;3) in rice

Yutaka Sonoda, Akira Ikeda, Satomi Saiki, Nicolaus Von Wirén, Tomoyuki Yamaya, Junji Yamaguchi

Research output: Contribution to journalArticlepeer-review

206 Citations (Scopus)

Abstract

To study the regulation of ammonium uptake into rice roots, three ammonium transporter genes (OsAMT1;1, 1;2 and 1;3; Oryza sativa ammonium transporter) were isolated and examined. OsAMT1s belong to AMT1 family, containing 11 putative transmembrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1-1;3 the complete AMT1 family of rice had been isolated. Heterologous expression of OsAMT1s in the yeast Saccharomyces cerevisiae mutant 31019b showed that all three OsAMT1s exhibit ammonium transport activity. Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3. In situ mRNA detection revealed that OsAMT1; 2 is expressed in the central cylinder and cell surface of root tips. This gene expression analysis revealed a distinct nitrogen-dependent regulation for AMTs in rice, differing from that in tomato or Arabidopsis.

Original languageEnglish
Pages (from-to)726-734
Number of pages9
JournalPlant and Cell Physiology
Volume44
Issue number7
DOIs
Publication statusPublished - 2003 Jul 1

Keywords

  • AMT1
  • Ammonium regulation
  • Ammonium uptake
  • In situ hybridization
  • Oryza sativa
  • Yeast complementation

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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