Distinct and overlapping roles of two gibberellin 3-oxidases in Arabidopsis development

Melissa G. Mitchum, Shinjiro Yamaguchi, Atsushi Hanada, Ayuko Kuwahara, Yasushi Yoshioka, Tomohiko Kato, Satoshi Tabata, Yuji Kamiya, Tai Ping Sun

    Research output: Contribution to journalArticlepeer-review

    194 Citations (Scopus)

    Abstract

    Gibberellin (GA) 3-oxidase, a class of 2-oxoglutarate-dependent dioxygenases, catalyzes the conversion of precursor GAs to their bioactive forms, thereby playing a direct role in determining the levels of bioactive GAs in plants. Gibberellin 3-oxidase in Arabidopsis is encoded by a multigene family consisting of at least four members, designated AtGA3ox1 to AtGA3ox4. It has yet to be investigated how each AtGA3ox gene contributes to optimizing bioactive GA levels during growth and development. Using quantitative real-time PCR analysis, we have shown that each AtGA3ox gene exhibits a unique organ-specific expression pattern, suggesting distinct developmental roles played by individual AtGA3ox members. To investigate the sites of synthesis of bioactive GA in plants, we generated transgenic Arabidopsis that carried AtGA3ox1-GUS and AtGA3ox2-GUS fusions. Comparisons of the GUS staining patterns of these plants with that of AtCPS-GUS from previous studies revealed the possible physical separation of the early and late stages of the GA pathway in roots. Phenotypic characterization and quantitative analysis of the endogenous GA content of ga3ox1 and ga3ox2 single and ga3ox1/ga3ox2 double mutants revealed distinct as well as overlapping roles of AtGA3ox1 and AtGA3ox2 in Arabidopsis development. Our results show that AtGA3ox1 and AtGA3ox2 are responsible for the synthesis of bioactive GAs during vegetative growth, but that they are dispensable for reproductive development. The stage-specific severe GA-deficient phenotypes of the ga3ox1/ga3ox2 mutant suggest that AtGA3ox3 and AtGA3ox4 are tightly regulated by developmental cues; AtGA3ox3 and AtGA3ox4 are not upregulated to compensate for GA deficiency during vegetative growth of the double mutant.

    Original languageEnglish
    Pages (from-to)804-818
    Number of pages15
    JournalPlant Journal
    Volume45
    Issue number5
    DOIs
    Publication statusPublished - 2006 Mar

    Keywords

    • Arabidopsis
    • Biosynthesis
    • Developmental regulation
    • GA 3-oxidases
    • GA mutants
    • Gibberellin

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

    Fingerprint Dive into the research topics of 'Distinct and overlapping roles of two gibberellin 3-oxidases in Arabidopsis development'. Together they form a unique fingerprint.

    Cite this