TY - JOUR
T1 - Distinct and essential roles of transcription factors IRF-3 and IRF-7 in response to viruses for IFN-α/β gene induction
AU - Sato, Mitsuharu
AU - Suemori, Hirofumi
AU - Hata, Naoki
AU - Asagiri, Masataka
AU - Ogasawara, Kouetsu
AU - Nakao, Kazuki
AU - Nakaya, Takeo
AU - Katsuki, Motoya
AU - Noguchi, Shigeru
AU - Tanaka, Nobuyuki
AU - Taniguchi, Tadatsugu
N1 - Funding Information:
We thank Drs. E. Barsoumian, S. Taki, S. Hida, and T. Yokochi for invaluable advice and Dr. H. Takeshima for providing pgk-hyg vector. This work was supported in part by a special grant for Advanced Research on Cancer from the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2000
Y1 - 2000
N2 - Induction of the interferon (IFN)-α/β gene transcription in virus-infected cells is an event central to innate immunity. Mice lacking the transcription factor IRF-3 are more vulnerable to virus infection. In embryonic fibroblasts, virus-induced IFN-α/β gene expression levels are reduced and the spectrum of the IFN-α mRNA subspecies altered. Furthermore, cells additionally defective in IRF-7 expression totally fail to induce these genes in response to infections by any of the virus types tested. In these cells, a normal profile of IFN-α/β mRNA induction can be achieved by coexpressing both IRF-3 and IRF-7. These results demonstrate the essential and distinct roles of the two factors, which together ensure the transcriptional efficiency and diversity of IFN-α/β genes for the antiviral response.
AB - Induction of the interferon (IFN)-α/β gene transcription in virus-infected cells is an event central to innate immunity. Mice lacking the transcription factor IRF-3 are more vulnerable to virus infection. In embryonic fibroblasts, virus-induced IFN-α/β gene expression levels are reduced and the spectrum of the IFN-α mRNA subspecies altered. Furthermore, cells additionally defective in IRF-7 expression totally fail to induce these genes in response to infections by any of the virus types tested. In these cells, a normal profile of IFN-α/β mRNA induction can be achieved by coexpressing both IRF-3 and IRF-7. These results demonstrate the essential and distinct roles of the two factors, which together ensure the transcriptional efficiency and diversity of IFN-α/β genes for the antiviral response.
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U2 - 10.1016/S1074-7613(00)00053-4
DO - 10.1016/S1074-7613(00)00053-4
M3 - Article
C2 - 11070172
AN - SCOPUS:0033680737
SN - 1074-7613
VL - 13
SP - 539
EP - 548
JO - Immunity
JF - Immunity
IS - 4
ER -
