TY - JOUR
T1 - Dissemination and proliferation of neural stem cells on the spinal cord by injection into the fourth ventricle of the rat
T2 - A method for cell transplantation
AU - Bai, Hongliang
AU - Suzuki, Yoshihisa
AU - Noda, Toru
AU - Wu, Sufan
AU - Kataoka, Kazuya
AU - Kitada, Masaaki
AU - Ohta, Masayoshi
AU - Chou, Hirotomi
AU - Ide, Chizuka
N1 - Funding Information:
The authors wish to thank the Takeda Science Foundation for its support. The authors also thank Professor M. Okabe, the Genome Information Research Center, Osaka University, for donating GFP-transgenic rats and Dr Tatsuo Arii (National Institute for Physiological Sciences, Center for Brain Experiment, Okazaki) for technical suggestions on HVEM. This study was supported by Grants-in-Aid 13357014, 14207073, 14571712 and 14657458 for scientific research from the Japanese Ministry of Science, Education and Culture.
PY - 2003/4/15
Y1 - 2003/4/15
N2 - We examined the distribution of hippocampus-derived neural stem cells on the spinal cord surface for up to 3 weeks following injection through the fourth ventricle. The injected cells were disseminated as tiny spots on the pia mater of the spinal cord and proliferated into large cell-clusters. On both the dorsal and ventral side, cell clusters increased in number rapidly up to 5 days after injection and thereafter decreased gradually due to the coalition of neighbouring clusters. Concomitantly, individual cell clusters continuously increased in size, occupying almost 50% of the spinal cord surface. Cell attachment was usually found around blood vessels, along which cells invaded into the spinal cord. In the injured site, cells migrated into the lesion and were integrated into the spinal cord tissue, some of which had differentiated into astrocytes 1-2 weeks after injection. BrdU-uptake experiments demonstrated that the transplanted cells proliferated within the host cerebrospinal fluid. These results indicate that application of neural stem cells through the ventricle is an effective method to disseminate cells all over the spinal cord and that they can migrate and be integrated into the injured spinal cord.
AB - We examined the distribution of hippocampus-derived neural stem cells on the spinal cord surface for up to 3 weeks following injection through the fourth ventricle. The injected cells were disseminated as tiny spots on the pia mater of the spinal cord and proliferated into large cell-clusters. On both the dorsal and ventral side, cell clusters increased in number rapidly up to 5 days after injection and thereafter decreased gradually due to the coalition of neighbouring clusters. Concomitantly, individual cell clusters continuously increased in size, occupying almost 50% of the spinal cord surface. Cell attachment was usually found around blood vessels, along which cells invaded into the spinal cord. In the injured site, cells migrated into the lesion and were integrated into the spinal cord tissue, some of which had differentiated into astrocytes 1-2 weeks after injection. BrdU-uptake experiments demonstrated that the transplanted cells proliferated within the host cerebrospinal fluid. These results indicate that application of neural stem cells through the ventricle is an effective method to disseminate cells all over the spinal cord and that they can migrate and be integrated into the injured spinal cord.
KW - Cerebrospinal fluid
KW - Fourth ventricle
KW - Green fluorescent protein (GFP)
KW - Neural stem cell
KW - Neurosphere
KW - Transplantation
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U2 - 10.1016/S0165-0270(03)00007-4
DO - 10.1016/S0165-0270(03)00007-4
M3 - Article
C2 - 12706848
AN - SCOPUS:0037446802
VL - 124
SP - 181
EP - 187
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
SN - 0165-0270
IS - 2
ER -