Disruption of the Microsomal glutathione S-transferase-like gene reduces life span of Drosophila melanogaster

Gakuta Toba, Toshiro Aigaki

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)


Microsomal glutathione S-transferase-I (MGST-I) has been thought to be important for protecting the cell from oxidative damages and/or xenobiotics. We have previously identified the Microsomal glutathione S-transferase-like (Mgstl) gene, a Drosophila homologue of human MGST-I. To investigate the function of the enzyme using Drosophila as a model system, we examined the expression pattern of Mgstl during development, and generated loss-of- function mutants to assess its in-vivo function. Mgstl was expressed in all developmental stages. It is expressed ubiquitously with the highest expression in the larval fat body, an insect organ thought to be functionally corresponding to mammalian liver, while relatively low in the central nervous system. This tissue distribution is consistent with that of MGST-I in humans or Rats. Mgstl null mutants generated from a P element insertion line showed no obvious defects in morphology, indicating that it is not essential for the development. However, their life span was significantly reduced compared to control flies, suggesting that the MGSTL protein is involved in processes somehow contributing to aging. We found an Mgstl pseudogene, which is apparently derived through the reverse transcription of Mgstl mRNA and subsequent integration into the genome. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)179-187
Number of pages9
Issue number2
Publication statusPublished - 2000 Aug 8
Externally publishedYes


  • Gene search system
  • Oxidative stress
  • P element excision
  • Pseudogene

ASJC Scopus subject areas

  • Genetics


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