Disruption of the APC Gene by a Retrotransposal Insertion of LI Sequence in a Colon Cancer

Yoshio Miki, Isamu Nishisho, Akira Horii, Yasuo Miyoshi, Yusuke Nakamura

Research output: Contribution to journalArticlepeer-review

392 Citations (Scopus)

Abstract

The APC gene is responsible for familial adenomatous polyposis and is considered to be a tumor suppressor gene associated with development of sporadic colorectal tumors. Here we report the disruption of the APC gene caused by somatic insertion of a long interspersed repetitive element (LINE-1 sequence) into the last exon of the APC gene in a colon cancer. The inserted sequence was composed of a 3′ portion of the LINE-1 consensus sequence and nearly 180 base pairs of polyadenylate tract. Furthermore, since an 8-base pair target site duplication was observed, retrotranscriptional insertion of an active LINE-1 sequence is suspected as the cause of this insertion event. This is the first report of the disruption of a tumor suppressor gene caused by somatic insertion of a mobile genetic element.

Original languageEnglish
Pages (from-to)643-645
Number of pages3
JournalCancer Research
Volume52
Issue number3
Publication statusPublished - 1992 Feb

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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