Disruption of ins-11, a Caenorhabditis elegans insulin-like gene, and phenotypic analyses of the gene-disrupted animal

Tsuyoshi Kawano; Ryosuke Nagatomo; Yasuo Kimura; Keiko Gengyo-Ando; Shohei Mitani

doi:10.1271/bbb.60472

Disruption of ins-11, a Caenorhabditis elegans insulin-like gene, and phenotypic analyses of the gene-disrupted animal

Tsuyoshi Kawano, Ryosuke Nagatomo, Yasuo Kimura, Keiko Gengyo-Ando, Shohei Mitani

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The insulin/insulin-like growth factor-I signaling (IIS) pathway regulates larval diapause, adult lifespan, fat metabolism, and stress-resistance in the nematode Caenorhabditis elegans. One of 38 C. elegans insulin-like genes, ins-11, was disrupted and phenotypic analyses of the gene-disrupted animal were performed. The gene-disruption exhibited a significant influence on the adult lifespan. It antagonized the lifespan extension induced by RNAi knockdown of another insulin-like gene, ins-7. Hence ins-11 appears to be necessary for lifespan extension caused by a decrease in the IIS pathway. This is the first description of gene-disruption of the C. elegans insulin-like gene that suppresses the lifespan extension.

Original language	English
Pages (from-to)	3084-3087
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	70
Issue number	12
DOIs	https://doi.org/10.1271/bbb.60472
Publication status	Published - 2006
Externally published	Yes

Keywords

Caenorhabditis elegans
Gene-disruption
Insulin-like gene
Lifespan regulation

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Disruption of ins-11, a Caenorhabditis elegans insulin-like gene, and phenotypic analyses of the gene-disrupted animal",

abstract = "The insulin/insulin-like growth factor-I signaling (IIS) pathway regulates larval diapause, adult lifespan, fat metabolism, and stress-resistance in the nematode Caenorhabditis elegans. One of 38 C. elegans insulin-like genes, ins-11, was disrupted and phenotypic analyses of the gene-disrupted animal were performed. The gene-disruption exhibited a significant influence on the adult lifespan. It antagonized the lifespan extension induced by RNAi knockdown of another insulin-like gene, ins-7. Hence ins-11 appears to be necessary for lifespan extension caused by a decrease in the IIS pathway. This is the first description of gene-disruption of the C. elegans insulin-like gene that suppresses the lifespan extension.",

keywords = "Caenorhabditis elegans, Gene-disruption, Insulin-like gene, Lifespan regulation",

author = "Tsuyoshi Kawano and Ryosuke Nagatomo and Yasuo Kimura and Keiko Gengyo-Ando and Shohei Mitani",

note = "Funding Information: The RNAi vector pPD.129.36 was kindly provided by Dr. Andrew Fire of the Carnegie Institute. The daf-2(e1370) mutant and E. coli HT115 strain were provided by the Caenorhabditis Genetic Center (CGC). We thank Dr. Shuji Honda and Dr. Yoko Honda of the Tokyo Metropolitan Institute for Gerontology for fruitful discussion. Some of the experiments in this study were carried out in the Venture Business Laboratory of Tottori University. This work was supported in part by a grant-in-aid to T.K. (no. 17580298) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan and a SUNBOR grant to T.K.",

year = "2006",

doi = "10.1271/bbb.60472",

language = "English",

volume = "70",

pages = "3084--3087",

journal = "Bioscience, Biotechnology and Biochemistry",

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AU - Nagatomo, Ryosuke

AU - Kimura, Yasuo

AU - Gengyo-Ando, Keiko

AU - Mitani, Shohei

N1 - Funding Information: The RNAi vector pPD.129.36 was kindly provided by Dr. Andrew Fire of the Carnegie Institute. The daf-2(e1370) mutant and E. coli HT115 strain were provided by the Caenorhabditis Genetic Center (CGC). We thank Dr. Shuji Honda and Dr. Yoko Honda of the Tokyo Metropolitan Institute for Gerontology for fruitful discussion. Some of the experiments in this study were carried out in the Venture Business Laboratory of Tottori University. This work was supported in part by a grant-in-aid to T.K. (no. 17580298) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan and a SUNBOR grant to T.K.

PY - 2006

Y1 - 2006

N2 - The insulin/insulin-like growth factor-I signaling (IIS) pathway regulates larval diapause, adult lifespan, fat metabolism, and stress-resistance in the nematode Caenorhabditis elegans. One of 38 C. elegans insulin-like genes, ins-11, was disrupted and phenotypic analyses of the gene-disrupted animal were performed. The gene-disruption exhibited a significant influence on the adult lifespan. It antagonized the lifespan extension induced by RNAi knockdown of another insulin-like gene, ins-7. Hence ins-11 appears to be necessary for lifespan extension caused by a decrease in the IIS pathway. This is the first description of gene-disruption of the C. elegans insulin-like gene that suppresses the lifespan extension.

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KW - Caenorhabditis elegans

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KW - Lifespan regulation

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Disruption of ins-11, a Caenorhabditis elegans insulin-like gene, and phenotypic analyses of the gene-disrupted animal

Abstract

Keywords

ASJC Scopus subject areas

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