Disruption of ins-11, a Caenorhabditis elegans insulin-like gene, and phenotypic analyses of the gene-disrupted animal

Tsuyoshi Kawano, Ryosuke Nagatomo, Yasuo Kimura, Keiko Gengyo-Ando, Shohei Mitani

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The insulin/insulin-like growth factor-I signaling (IIS) pathway regulates larval diapause, adult lifespan, fat metabolism, and stress-resistance in the nematode Caenorhabditis elegans. One of 38 C. elegans insulin-like genes, ins-11, was disrupted and phenotypic analyses of the gene-disrupted animal were performed. The gene-disruption exhibited a significant influence on the adult lifespan. It antagonized the lifespan extension induced by RNAi knockdown of another insulin-like gene, ins-7. Hence ins-11 appears to be necessary for lifespan extension caused by a decrease in the IIS pathway. This is the first description of gene-disruption of the C. elegans insulin-like gene that suppresses the lifespan extension.

Original languageEnglish
Pages (from-to)3084-3087
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number12
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • Caenorhabditis elegans
  • Gene-disruption
  • Insulin-like gene
  • Lifespan regulation

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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