Dispersion and degradation of environmental DNA from caged fish in a marine environment

Hiroaki Murakami, Seokjin Yoon, Akihide Kasai, Toshifumi Minamoto, Satoshi Yamamoto, Masayuki K. Sakata, Tomoya Horiuchi, Hideki Sawada, Michio Kondoh, Yoh Yamashita, Reiji Masuda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Environmental DNA (eDNA) consists of DNA fragments shed from organisms into the environment, and can be used to identify species presence and abundance. This study aimed to reveal the dispersion and degradation processes of eDNA in the sea. Caged fish were set off the end of a pier in Maizuru Bay, the Sea of Japan, and their eDNA was traced at sampling stations located at the cage and 10, 30, 100, 300, 600 and 1000 m distances from the cage along two transect lines. Sea surface water was collected at each station at 0, 2, 4, 8, 24 and 48 h after setting the cage, and again after removing the cage. Quantitative PCR analyses using a species-specific primer and probe set revealed that the target DNA was detectable while the cage was present and for up to 1 h after removing the cage, but not at 2 h or later. Among the 57 amplified samples, 45 (79%) were collected within 30 m from the cage. These results suggest that eDNA can provide a snapshot of organisms present in a coastal marine environment.

Original languageEnglish
Pages (from-to)327-337
Number of pages11
JournalFisheries Science
Volume85
Issue number2
DOIs
Publication statusPublished - 2019 Mar 15

Keywords

  • Detectability
  • Quantitative PCR
  • Species-specific primers and probe
  • Striped jack Pseudocaranx dentex
  • Transport
  • eDNA

ASJC Scopus subject areas

  • Aquatic Science

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    Murakami, H., Yoon, S., Kasai, A., Minamoto, T., Yamamoto, S., Sakata, M. K., Horiuchi, T., Sawada, H., Kondoh, M., Yamashita, Y., & Masuda, R. (2019). Dispersion and degradation of environmental DNA from caged fish in a marine environment. Fisheries Science, 85(2), 327-337. https://doi.org/10.1007/s12562-018-1282-6