Disperse Orange 3 as a resonance Raman probe for measuring membrane order

Yuki Numakura; Takashi Miura

doi:10.1016/j.fob.2015.10.008

Disperse Orange 3 as a resonance Raman probe for measuring membrane order

Yuki Numakura, Takashi Miura

PHA - Molecular Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

Abstract

Resonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO₂ symmetric stretching band of 4-(4-nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4cm^-1 on the phase transition of phosphatidylcholine membranes from the liquid crystalline to the gel phase. A comparable downshift also occurs when DO3 is bound to cholesterol-containing membranes in the liquid-ordered phase. Our results demonstrate that Raman spectrum of DO3 is a unique tool for measuring the molecular order of lipids in membranes.

Original language	English
Pages (from-to)	859-863
Number of pages	5
Journal	FEBS Open Bio
Volume	5
DOIs	https://doi.org/10.1016/j.fob.2015.10.008
Publication status	Published - 2015

Keywords

Disperse orange 3
Membrane microdomains
Membrane order
Raman spectroscopy
Resonance raman probe

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.fob.2015.10.008

Cite this

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title = "Disperse Orange 3 as a resonance Raman probe for measuring membrane order",

abstract = "Resonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO2 symmetric stretching band of 4-(4-nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4cm-1 on the phase transition of phosphatidylcholine membranes from the liquid crystalline to the gel phase. A comparable downshift also occurs when DO3 is bound to cholesterol-containing membranes in the liquid-ordered phase. Our results demonstrate that Raman spectrum of DO3 is a unique tool for measuring the molecular order of lipids in membranes.",

keywords = "Disperse orange 3, Membrane microdomains, Membrane order, Raman spectroscopy, Resonance raman probe",

author = "Yuki Numakura and Takashi Miura",

note = "Funding Information: This study was supported in part by Grants-in-Aid for Scientific Research (Grant 24590043 ) from Japan Society for the Promotion of Science . We are also grateful to Prof. Takakazu Nakabayashi (Tohoku University) for fruitful discussion. Publisher Copyright: {\textcopyright} 2015 The Authors.",

year = "2015",

doi = "10.1016/j.fob.2015.10.008",

language = "English",

volume = "5",

pages = "859--863",

journal = "FEBS Open Bio",

issn = "2211-5463",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Disperse Orange 3 as a resonance Raman probe for measuring membrane order

AU - Numakura, Yuki

AU - Miura, Takashi

N1 - Funding Information: This study was supported in part by Grants-in-Aid for Scientific Research (Grant 24590043 ) from Japan Society for the Promotion of Science . We are also grateful to Prof. Takakazu Nakabayashi (Tohoku University) for fruitful discussion. Publisher Copyright: © 2015 The Authors.

PY - 2015

Y1 - 2015

N2 - Resonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO2 symmetric stretching band of 4-(4-nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4cm-1 on the phase transition of phosphatidylcholine membranes from the liquid crystalline to the gel phase. A comparable downshift also occurs when DO3 is bound to cholesterol-containing membranes in the liquid-ordered phase. Our results demonstrate that Raman spectrum of DO3 is a unique tool for measuring the molecular order of lipids in membranes.

AB - Resonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO2 symmetric stretching band of 4-(4-nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4cm-1 on the phase transition of phosphatidylcholine membranes from the liquid crystalline to the gel phase. A comparable downshift also occurs when DO3 is bound to cholesterol-containing membranes in the liquid-ordered phase. Our results demonstrate that Raman spectrum of DO3 is a unique tool for measuring the molecular order of lipids in membranes.

KW - Disperse orange 3

KW - Membrane microdomains

KW - Membrane order

KW - Raman spectroscopy

KW - Resonance raman probe

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JO - FEBS Open Bio

JF - FEBS Open Bio

SN - 2211-5463

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Disperse Orange 3 as a resonance Raman probe for measuring membrane order

Abstract

Keywords

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