Dislocations in high-quality glucose isomerase crystals grown from seed crystals

H. Koizumi, M. Tachibana, I. Yoshizaki, S. Fukuyama, K. Tsukamoto, Y. Suzuki, S. Uda, K. Kojima

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Glucose isomerase crystals with thicknesses of 0.5 mm were grown using seed crystals. The thickness required for better X-ray topographic images by kinematical theory is determined by the extinction distance, g. The seed crystals were cross-linked with glutaraldehyde to maintain stability for a long period before the growth experiments. Dislocations that were produced during the growth of such thick glucose isomerase crystals were observed as clearly as those in inorganic crystals via X-ray topography with monochromatic-beam synchrotron radiation, which is attributed to the high quality of the glucose isomerase crystals. This also indicates that high-quality protein crystals can be grown using seed crystals. The dislocations extended from the interface with the cross-linked seed crystal to the surface of the grown crystal; however, no dislocations were generated if the seed crystal was not cross-linked. We discuss the effect of the chemical cross-linking of the seed on the generation of dislocations from seed crystals.

Original languageEnglish
Pages (from-to)5111-5116
Number of pages6
JournalCrystal Growth and Design
Volume14
Issue number10
DOIs
Publication statusPublished - 2014 Oct 1

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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