Abstract
Glucose isomerase crystals with thicknesses of 0.5 mm were grown using seed crystals. The thickness required for better X-ray topographic images by kinematical theory is determined by the extinction distance, g. The seed crystals were cross-linked with glutaraldehyde to maintain stability for a long period before the growth experiments. Dislocations that were produced during the growth of such thick glucose isomerase crystals were observed as clearly as those in inorganic crystals via X-ray topography with monochromatic-beam synchrotron radiation, which is attributed to the high quality of the glucose isomerase crystals. This also indicates that high-quality protein crystals can be grown using seed crystals. The dislocations extended from the interface with the cross-linked seed crystal to the surface of the grown crystal; however, no dislocations were generated if the seed crystal was not cross-linked. We discuss the effect of the chemical cross-linking of the seed on the generation of dislocations from seed crystals.
Original language | English |
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Pages (from-to) | 5111-5116 |
Number of pages | 6 |
Journal | Crystal Growth and Design |
Volume | 14 |
Issue number | 10 |
DOIs | |
Publication status | Published - 2014 Oct 1 |
ASJC Scopus subject areas
- Chemistry(all)
- Materials Science(all)
- Condensed Matter Physics