Disinfection efficacy and mechanism of olanexidine gluconate against norovirus

Kaoru Imai, Makoto Tanaka, Seiji Miyoshi, Ren Murakami, Akifumi Hagi, Sachi Yamagawa, Daisuke Sano

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Background: The purpose of this study was to evaluate the virucidal activity of a new olanexidine-containing formulation for hand hygiene (olanexidine gluconate hand rub; OLG-HR) against non-enveloped viruses and to understand its mechanism of action. Methods: The virucidal activities of OLG-HR against two strains of caliciviruses and three adenovirus serotypes were evaluated through suspension tests. Also, virus-like particles were used to predict the effect of olanexidine gluconate on virus particle structure. Results: The results of suspension tests under conditions with and without interfering substances (1.5% BSA) indicated that OLG-HR had a broad-spectrum effect against non-enveloped viruses, and the virucidal effect was unaffected by organic contaminants. Furthermore, olanexidine inhibited the binding ability of virus-like particles to the binding receptor of human norovirus and increased the aggregation of virus-like particles in a dose-dependent manner. Transmission electron microscopy showed that the morphology of the virus-like particles was affected by exposure to olanexidine, indicating that the protein-denaturing effect of olanexidine gluconate caused the loss of receptor-binding capability of the viral capsid protein. Conclusions: This study suggests that olanexidine gluconate is a potential biological and environmental disinfectant against norovirus and adenovirus.

Original languageEnglish
Pages (from-to)764-771
Number of pages8
JournalAmerican Journal of Infection Control
Issue number7
Publication statusPublished - 2022 Jul


  • Adenovirus
  • Antiseptic
  • Non-enveloped virus
  • Norovirus
  • Olanexidine
  • Virus-like particle

ASJC Scopus subject areas

  • Epidemiology
  • Health Policy
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases


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