Disassembly-driven turn-on sensing of enzyme activity by substrate-based fluorescent nanoprobe

Yousuke Takaoka; Yoshiaki Fukuyama; Kazuya Matsuo; Itaru Hamachi

doi:10.1246/cl.130697

Disassembly-driven turn-on sensing of enzyme activity by substrate-based fluorescent nanoprobe

Yousuke Takaoka, Yoshiaki Fukuyama, Kazuya Matsuo, Itaru Hamachi

Research output: Contribution to journal › Review article › peer-review

2 Citations (Scopus)

Abstract

Herein, we report a novel signal-amplifiable self-assembling fluorescent nanoprobe for turn-on detection of specific enzyme activity. This probe employed a substrate of matrix metalloproteinase 2 (MMP2) together with a self-assembling unit, and showed a detection limit of less than 830 pM, which is 84-fold lower than that of our previous ligand-tethered fluorescent nanoprobes. Using this new probe, we succeeded in monitoring the specific activity of secreted MMP even in the conditioned media of tumor cells.

Original language	English
Pages (from-to)	1426-1428
Number of pages	3
Journal	Chemistry Letters
Volume	42
Issue number	11
DOIs	https://doi.org/10.1246/cl.130697
Publication status	Published - 2013
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)

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10.1246/cl.130697

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abstract = "Herein, we report a novel signal-amplifiable self-assembling fluorescent nanoprobe for turn-on detection of specific enzyme activity. This probe employed a substrate of matrix metalloproteinase 2 (MMP2) together with a self-assembling unit, and showed a detection limit of less than 830 pM, which is 84-fold lower than that of our previous ligand-tethered fluorescent nanoprobes. Using this new probe, we succeeded in monitoring the specific activity of secreted MMP even in the conditioned media of tumor cells.",

author = "Yousuke Takaoka and Yoshiaki Fukuyama and Kazuya Matsuo and Itaru Hamachi",

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T1 - Disassembly-driven turn-on sensing of enzyme activity by substrate-based fluorescent nanoprobe

AU - Takaoka, Yousuke

AU - Fukuyama, Yoshiaki

AU - Matsuo, Kazuya

AU - Hamachi, Itaru

PY - 2013

Y1 - 2013

N2 - Herein, we report a novel signal-amplifiable self-assembling fluorescent nanoprobe for turn-on detection of specific enzyme activity. This probe employed a substrate of matrix metalloproteinase 2 (MMP2) together with a self-assembling unit, and showed a detection limit of less than 830 pM, which is 84-fold lower than that of our previous ligand-tethered fluorescent nanoprobes. Using this new probe, we succeeded in monitoring the specific activity of secreted MMP even in the conditioned media of tumor cells.

AB - Herein, we report a novel signal-amplifiable self-assembling fluorescent nanoprobe for turn-on detection of specific enzyme activity. This probe employed a substrate of matrix metalloproteinase 2 (MMP2) together with a self-assembling unit, and showed a detection limit of less than 830 pM, which is 84-fold lower than that of our previous ligand-tethered fluorescent nanoprobes. Using this new probe, we succeeded in monitoring the specific activity of secreted MMP even in the conditioned media of tumor cells.

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U2 - 10.1246/cl.130697

DO - 10.1246/cl.130697

M3 - Review article

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JO - Chemistry Letters

JF - Chemistry Letters

SN - 0366-7022

IS - 11

ER -

Disassembly-driven turn-on sensing of enzyme activity by substrate-based fluorescent nanoprobe

Abstract

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