Disassembly-driven turn-on sensing of enzyme activity by substrate-based fluorescent nanoprobe

Yousuke Takaoka, Yoshiaki Fukuyama, Kazuya Matsuo, Itaru Hamachi

Research output: Contribution to journalReview articlepeer-review

2 Citations (Scopus)


Herein, we report a novel signal-amplifiable self-assembling fluorescent nanoprobe for turn-on detection of specific enzyme activity. This probe employed a substrate of matrix metalloproteinase 2 (MMP2) together with a self-assembling unit, and showed a detection limit of less than 830 pM, which is 84-fold lower than that of our previous ligand-tethered fluorescent nanoprobes. Using this new probe, we succeeded in monitoring the specific activity of secreted MMP even in the conditioned media of tumor cells.

Original languageEnglish
Pages (from-to)1426-1428
Number of pages3
JournalChemistry Letters
Issue number11
Publication statusPublished - 2013
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)


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