Direct visualization of synthetic cellulose formation via enzymatic polymerization using transmission electron microscopy

Shiro Kobayashi, Shin‐ichiro ‐i Shoda, Jong Lee, Kazuo Okuda, R. Malcolm Brown, Shigenori Kuga

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The process of synthetic cellulose formation in an acetonitrile‐buffer solvent system by enzymatic polymerization of β‐cellobiosyl fluoride monomer using cellulase as a catalyst was visualized by transmission electron microscopy. Synthetic cellulose could be observed as soon as 30 s after initiation of the polymerization. A micellar phase separation occurred at the initial stage of the reaction. Irregular aggregates of cellulose were formed at the boundary of the micellar particles, suggesting that the interface is the site of polymerization. The cellulose product after treatment with hot surfactant solution was strongly labeled with cellobiohydrolase I‐gold complexes, indicating that the produced polymer is cellulose. The electron diffraction pattern of the product showed the typical pattern of crystal structure of cellulose II.

Original languageEnglish
Pages (from-to)1319-1326
Number of pages8
JournalMacromolecular Chemistry and Physics
Volume195
Issue number4
DOIs
Publication statusPublished - 1994 Apr

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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