Direct plasma stimuli including electrostimulation and OH radical induce transient increase in intracellular Ca2+ and uptake of a middle-size membrane-impermeable molecule

Shota Sasaki, Yutaro Hokari, Akiko Kumada, Makoto Kanzaki, Toshiro Kaneko

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Despite the promising potential of medical treatments based on non-equilibrium atmospheric-pressure plasma, most of the underlying mechanisms remain unknown. Here we report that direct plasma irradiation (DPI) can induce transient increase in intracellular calcium (Ca2+) concentrations, thereby playing key roles in many cellular processes as well as the uptake of middle-size membrane-impermeable molecules, which did not occur with indirect plasma irradiation (IPI). Experimental measurements using a Pockels voltage probe and gelling chemical reagent showed that electric field of 150 kV/cm and hydroxyl radical (OH) supply to cell cultures were induced by DPI under a typical condition compared with IPI. In addition, significant inhibition of the OH scavenger D-mannitol indicated involvement of OH in those DPI-induced cellular responses. These results facilitate the elucidation of plasma-cell interaction and the development of plasma devices for medical treatments.

Original languageEnglish
Article number1700077
JournalPlasma Processes and Polymers
Volume15
Issue number1
DOIs
Publication statusPublished - 2018 Jan

Keywords

  • OH radical
  • Pockels cell
  • atmospheric pressure plasma
  • membrane transport
  • non-thermal plasma

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Polymers and Plastics

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