Direct observation of specific interaction between enzyme-substrate complexes using colloidal probe atomic force microscopy

Takehiro Suzuki, Yuan Wei Zhang, Tanetoshi Koyama, Darryl Y. Sasaki, Kazue Kurihara

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The interactions between the enzyme-substrate complex of heptaprenyl diphosphate synthase were directly investigated using colloidal probe atomic force microscopy. This enzyme is composed of two dissociable subunits which exhibit a catalytic activity only when they are associated together in the presence of Mg2+ and farnesyl diphosphate (FPP). We observed the adhesive force between the subunits only in the presence of both Mg2+ (0.1 mM) and FPP (15 μM). This is the first direct demonstration of the specific interaction involved in the enzyme reaction.

Original languageEnglish
Pages (from-to)536-537
Number of pages2
JournalChemistry Letters
Volume33
Issue number5
DOIs
Publication statusPublished - 2004 May 5

ASJC Scopus subject areas

  • Chemistry(all)

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