@article{55b3206b2e5145e49b20eeb13f3dfa7e,
title = "Direct observation of binding between biotinylated okadaic acids and protein phosphatase 2A monitored by surface plasmon resonance",
abstract = "Two biotin conjugates of okadaic acid were synthesized for evaluating their interactions with protein phosphatase 2A by surface plasmon resonance (SPR). C7-biotinylated okadaic acid revealed strong binding affinity to the enzyme, while C1-biotinylated derivative being devoid of affinity, implying that the C7-biotin conjugate is a useful tool for biochemical studies of protein phosphatase 2A.",
keywords = "Okadaic acid, Protein phosphatases, Surface plasmon resonance",
author = "Keiichi Konoki and Naoyuki Sugiyama and Michio Murata and Kazuo Tachibana",
note = "Funding Information: Acknowledgment : This work is supported by Grant-In-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan, and by Special Coordination Funds from the Science and Technology Agency Japan (Asian Network on Microbial Research). The authors are grateful to Dr. M. Ojika of Nagoya University for discussing the derivatization of okadalc acid, to Dr. M. Sasaki in our laboratory for discussion and critical reading of the manuscript, to Dr. J. Inagawa of Biacore AB for valuable discussions and suggestions, and to Messrs. H. Kusuoku, S. Matsuoka, H. Hori, and K. Otsuki in our laboratory for their help in collecting H. okadai.",
year = "1999",
month = jan,
day = "29",
doi = "10.1016/S0040-4039(98)02474-5",
language = "English",
volume = "40",
pages = "887--890",
journal = "Tetrahedron Letters",
issn = "0040-4039",
publisher = "Elsevier Limited",
number = "5",
}