Direct measurement of phagolysosomal esterase activity

Takafumi Uchida, Shuntaro Hosaka, Kumiko Miura

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A new method of directly measuring esterase activity within phagolysosomes has been developed. Decanoyl fluorescein- binding microspheres were prepared and phagocytosed by human peripheral neutrophils. Within phagolysoscmes lysosomal esterase hydrolyzed decanoyl fluorescein on the microspheres, causing the conversion of decanoyl fluorescein- binding microspheres (non-fluorescent) into fluorescein- binding microspheres (fluorescent). The activity of phagolysosomal esterase in intact neutrophils was assayed by the measurement of the fluorescence intensity without rupturing cells. By use of a flow cytometer, esterase activity within phagolysoscmes in single cells was measured.

Original languageEnglish
Pages (from-to)584-589
Number of pages6
JournalBiochemical and biophysical research communications
Volume127
Issue number2
DOIs
Publication statusPublished - 1985 Mar 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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