Direct link between Atg protein and small GTPase Rab: Atg16L functions as a potential Rab33 effector in mammals

Mitsunori Fukuda, Takashi Itoh

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Atg16L is a factor that is essential for elongation of the isolation membrane (also called phagophore), a precursor of the autophagosome. Atg16L facilitates LC3/Atg8-conjugation to phosphatidylethanolamine by forming an oligomeric complex with Atg12-conjugated Atg5 and recruiting an LC3-Atg3 intermediate to elongating isolation membranes. Although Atg16L is responsible for the isolation membrane localization of the complex, the mechanism by which Atg16L is targeted to or recognizes isolation membranes remains largely unknown. We recently reported finding that Atg16L specifically and directly interacts with the Golgi-resident small GTPase Rab33B (and Rab33A) via the coiled-coil domain of Atg16L. Since expression of a GTPase-deficient mutant of Rab33B or the coiled-coil domain of Atg16L modulates macroautophagy (simply referred to as autophagy below), Atg16L (or the Atg12-5/16L complex) is likely to function as a specific effector molecule for Rab33 in autophagosome formation. Future study of the cross talk between Atg16L-mediated autophagosome formation and Rab33-mediated membrane trafficking should provide an important clue to unresolved issues in autophagosome formation, specifically, the membrane source of autophagosomes.

Original languageEnglish
Pages (from-to)824-826
Number of pages3
JournalAutophagy
Volume4
Issue number6
DOIs
Publication statusPublished - 2008 Aug 16

Keywords

  • Atg16L
  • Autophagosome
  • Golgi
  • Isolation membrane/phagophore
  • LC3/Atg8
  • Membrane traffic
  • Rab effector
  • Rab33

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Direct link between Atg protein and small GTPase Rab: Atg16L functions as a potential Rab33 effector in mammals'. Together they form a unique fingerprint.

  • Cite this