Direct interaction of ligand-receptor pairs specifying stomatal patterning

Jin Suk Lee, Takeshi Kuroha, Marketa Hnilova, Dmitriy Khatayevich, Masahiro M. Kanaoka, Jessica M. Mcabee, Mehmet Sarikaya, Candan Tamerler, Keiko U. Torii

    Research output: Contribution to journalArticlepeer-review

    233 Citations (Scopus)


    Valves on the plant epidermis called stomata develop according to positional cues, which likely involve putative ligands (EPIDERMAL PATTERNING FACTORS [EPFs]) and putative receptors (ERECTA family receptor kinases and TOO MANY MOUTHS [TMM]) in Arabidopsis. Here we report the direct, robust, and saturable binding of bioactive EPF peptides to the ERECTA family. In contrast, TMM exhibits negligible binding to EPF1 but binding to EPF2. The ERECTA family forms receptor homomers in vivo. On the other hand, TMM associates with the ERECTA family but not with itself. While ERECTA family receptor kinases exhibit complex redundancy, blocking ERECTA and ERECTA-LIKE1 (ERL1) signaling confers specific insensitivity to EPF2 and EPF1, respectively. Our results place the ERECTA family as the primary receptors for EPFs with TMM as a signal modulator and establish EPF2-ERECTA and EPF1-ERL1 as ligand-receptor pairs specifying two steps of stomatal development: initiation and spacing divisions.

    Original languageEnglish
    Pages (from-to)126-136
    Number of pages11
    JournalGenes and Development
    Issue number2
    Publication statusPublished - 2012 Jan 15


    • Biochemical interaction
    • Cell type differentiation
    • Peptide ligands
    • Plant biology
    • Receptor kinases
    • Stomatal development
    • Tissue patterning

    ASJC Scopus subject areas

    • Genetics
    • Developmental Biology


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