Direct injection method for quantitation of endogenous leukotriene E 4 in human urine by liquid chromatography/electrospray ionization tandem mass spectrometry with a column-switching technique

N. Kishi, N. Mano, N. Asakawa

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

A new analytical method employing liquid chromatography/tandem mass spectrometry (LC/MS/MS) with a column-switching system was developed for quantitative determination of leukotriene E 4 (LTE 4 ) in human urine. A column-switching system using a trapping column, which concentrates the analyte and removes salts and other water-soluble contaminants, allowed direct injection of human urine. Because simultaneously eluted endogenous contaminants suppressed the ionization efficiency of LTE 4 , good liquid chromatographic separation was very important for establishing this method, notwithstanding the high selectivity of MS/MS. The calibration curve was linear over the range from 10 to 3000 pg/mL, and the method showed good accuracy and precision. This method should therefore be very useful for determination of LTF 4 amounts in human urine in studies on leukotriene metabolism and the efficacy of antileukotriene drugs.

Original languageEnglish
Pages (from-to)709-713
Number of pages5
Journalanalytical sciences
Volume17
Issue number6
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Direct injection method for quantitation of endogenous leukotriene E <sub>4</sub> in human urine by liquid chromatography/electrospray ionization tandem mass spectrometry with a column-switching technique'. Together they form a unique fingerprint.

Cite this