Direct in vivo protein transduction into a specific restricted brain area in rats

Kyoko Owada-Makabe, Yuji Tsubota, Kazunori Yukawa, Nobuyuki Kakimoto, Xiang Miang Liang, Masakazu Ichinose, Masanobu Maeda

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Attempts at protein transduction into specific restricted brain areas have remained unsuccessful. We attempted targeted, direct in vivo protein transduction by microinjecting β-galactosidase (β-gal) with hemagglutinating virus of Japan envelope (HVJ-E) vector into the rat nucleus tractus solitarius (NTS). The medulla oblongata including the NTS was removed 6 h post-injection and cryostat sections were histochemically stained to detect β-gal enzymatic activity. β-gal-positive cells were present in these sections as was β-gal activity determined by colorimetric analysis. β-gal-positive cells were not present in the rats microinjected only β-gal protein without HVJ-E vector. Our findings suggest that direct in vivo protein transduction into specific restricted brain areas is possible. The type of targeted delivery system we present may have wide applications in the administration of therapeutic proteins to the central nervous system.

Original languageEnglish
Pages (from-to)18-21
Number of pages4
JournalNeuroscience Letters
Volume378
Issue number1
DOIs
Publication statusPublished - 2005 Apr 11
Externally publishedYes

Keywords

  • Brain
  • Microinjection
  • Non-viral vector
  • Nucleus tractus solitarius
  • Protein therapy
  • Protein transduction

ASJC Scopus subject areas

  • Neuroscience(all)

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