Direct evidence of in vivo nitric oxide production and inducible nitric oxide synthase mRNA expression in the brain of living rat during experimental meningitis

Yasuhiro Suzuki, Satoshi Fujii, Teiji Tominaga, Takashi Yoshimoto, Shigemoto Fujii, Takaaki Akaike, Hiroshi Maeda, Tetsuhiko Yoshimura

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

To detect endogenous nitric oxide (NO) produced in a rat bacterial meningitis model, the authors applied an electron paramagnetic resonance (EPR) NO-trapping technique. Iron complex with N,N-diethyldithiocarbamate were used as a trapping agent. Experimental meningitis was induced by a mixture of lipopolysaccharide and interferon-γ. Sequential changes of NO formation under meningitis were observed in rat brain tissue by using X-band (9 GHz) EPR spectroscopy, and endogenous NO was detected in the head of a living rat with a 700-MHz EPR system. Inducible NO synthase mRNA expression in the brain tissues also was proven by using a reverse transcriptase- polymerase chain reaction technique.

Original languageEnglish
Pages (from-to)1175-1178
Number of pages4
JournalJournal of Cerebral Blood Flow and Metabolism
Volume19
Issue number11
DOIs
Publication statusPublished - 1999

Keywords

  • In vivo electron paramagnetic resonance detection
  • Inducible nitric oxide synthase mRNA
  • Meningitis
  • Nitric oxide
  • Spin-Trapping

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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