Direct evidence for specific binding of the replicative origin of the Escherichia coli chromosome to the membrane

Tomonobu Kusano, D. Steinmetz, W. G. Hendrickson, J. Murchie, M. King, A. Benson, M. Schaechter

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    The origin of replication of the Escherichia coli chromosomal DNA binds with high affinity to outer membrane preparations. This specific binding requires a 463-base-pair region of origin DNA between positions -45 and +417 of the oriC map. We show that binding does not require the presence of adjacent regions. From further analysis, we conclude that more than one binding site resides within the 325-base-pair fragment between positions +38 (BamHI) and +417 (XhoI). When this fragment is cut, two pieces bind with high affinity and one binds with lesser affinity. The binding ability of one of the high affinity sites is abolished by cutting it at position +92 with BamHI.

    Original languageEnglish
    Pages (from-to)313-316
    Number of pages4
    JournalJournal of bacteriology
    Volume158
    Issue number1
    Publication statusPublished - 1984 Jun 1

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology

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  • Cite this

    Kusano, T., Steinmetz, D., Hendrickson, W. G., Murchie, J., King, M., Benson, A., & Schaechter, M. (1984). Direct evidence for specific binding of the replicative origin of the Escherichia coli chromosome to the membrane. Journal of bacteriology, 158(1), 313-316.