Direct electroporation of adherent cells by hydrogel-based microelectrodes

M. Nishizawa, K. Nagamine

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a technique to fabricate Au microelectrodes on hydrogel substrates such as polyacrylamide, agarose, collagen etc. Electropolymerized poly (3,4-ethylenedioxythiophene) (PEDOT) serves as the anchor for bonding the Au electrodes to the hydrogels. The prepared soft hydrogel-based electrode can be directly laminated to adherent cells for in-situ electroporation. Owing to the molecular permeability of the hydrogels, continuous post-cultivation with the laminated electrode substrate was also possible. The electroporation- assisted uptakes of dye molecules and genes were demonstrated for normal human dermal fibroblast cells and human iPS cells.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1734-1736
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Keywords

  • Adherent cell
  • Electroporation
  • GFP
  • Hydrogel

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Nishizawa, M., & Nagamine, K. (2013). Direct electroporation of adherent cells by hydrogel-based microelectrodes. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1734-1736). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3). Chemical and Biological Microsystems Society.