Direct determination of serum glycyrrhetic acid by a monoclonal antibody-based inhibition ELISA using ibuprofen for releasing serum albumin- bound glycyrrhetic acid

Kunihiko Itoh, Masataka Hayasaka, Masahiro Niizeki, Seishiro Nozaki, Shunji Ishiwata, Yoshihisa Tomioka, Michinao Mizugaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We found that ibuprofen (IBU) had a potential for releasing serum albumin-bound glycyrrhetic acid (GA). Based on this observation, IBU was used to pretreat samples for the determination of serum GA levels by an inhibition ELISA. This method, termed IBU method was evaluated by the recovery of GA from human serum albumin (HSA) or normal human serum (NHS) that contained the exogeneously added GA (37-1000 ng/ml). The mean recovery of GA from HSA and NHS samples treated with IBU were 104.7 and 105.2%, respectively, whereas those without IBU pretreatment were 2.8 and 10.7%, respectively. Comparison of IBU method and chloroform extraction method revealed that the GA content of serum samples pretreated by each method were almost the same. These results suggest that IBU method is useful as a serum processing procedure for the determination of serum GA levels by an inhibition ELISA.

Original languageEnglish
Pages (from-to)343-352
Number of pages10
JournalJournal of Immunoassay
Volume17
Issue number4
DOIs
Publication statusPublished - 1996 Jan 1

Keywords

  • drug monitoring
  • glycyrrhetic acid
  • human serum
  • ibuprofen
  • inhibition ELISA

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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