Direct Detection of External Charge in Bacteriorhodospin by X-Ray Photoelectron Spectroscopy

Masae Takahashi, Takashi Takahashi, Kentaro Murano

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

X-ray photoelectron spectroscopy has been applied for 13-(trifluoromethyl)-bacteriorhodopsin in order to study the electrostatic environment of retinylidene moiety in bacteriorhodopsin. It has been found that the F Is binding energy of the pigment is about 3.4 eV smaller than that of free 13-(trifluoromethyl)retinal. This is the first and direct experimental detection of an external negative charge characterizing the optical properties of bacteriorhodopsin. The numerical interpretation of the chemical shift suggests that the negative charge is situated about 3 A distant from the C13-methyl group of a retinal molecule.

Original languageEnglish
Pages (from-to)3321-3323
Number of pages3
Journaljournal of the physical society of japan
Volume53
Issue number10
DOIs
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Direct Detection of External Charge in Bacteriorhodospin by X-Ray Photoelectron Spectroscopy'. Together they form a unique fingerprint.

Cite this