Direct demonstration of amino acid sequence recognition between antigen and antibody using surface forces measurement

Takehiro Suzuki, Takeshi Nakanishi, Kouhei Tsumoto, Izumi Kumagai, Kazue Kurihara

Research output: Contribution to conferencePaperpeer-review

Abstract

Changes in the interactions between an antigen and antibody upon single amino acid mutation were directly investigated using colloidal probe atomic force microscopy. Interactions studied were those between hen egg-white Lysozyme (Lysozyme) and HyHEL 10 scFv that is an antibody of Lysozyme and a mutant of the Lysozyme, K97M and K97A. K97M has a small association constant with HyHEL 10 scFv, whilst K97A doesn't associate with Lysozyme. We observed the adhesive force between HyHEL 10 scFv / Lysozyme and between HyHEL 10 scFv / K97M, and the former was larger than the latter. No adhesive force between HyHEL 10 scFv / K97A was observed. This is the first direct demonstration of the changes in the interactions upon a single amino acid mutation.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Keywords

  • Colloidal probe atomic force microscopy
  • Direct measurement
  • Single amino acid mutation

ASJC Scopus subject areas

  • Engineering(all)

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