Direct conversion of starch into glycolipid by using Thermoactinomyces vulgaris R-47 α-amylase

Kenji Yamamoto; Atsushi Kobayashi; Hiroshi Ito; Takashi Tonozuka; Yoshiyuki Sakano; Shin Ichiro Shoda

Direct conversion of starch into glycolipid by using Thermoactinomyces vulgaris R-47 α-amylase

Kenji Yamamoto, Atsushi Kobayashi, Hiroshi Ito, Takashi Tonozuka, Yoshiyuki Sakano, Shin Ichiro Shoda

ENG - Biomolecular Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

We have reported that irradiation of ultrasound onto substrate mixture containing starch and glycolipid enhanced the transglycosylating activity of commercial α-amylase preparation. In this paper, we demonstrated a glycosyl-transferring reaction to various glycosyl acceptors employing α-amylase from Thermoactinomyces vulgaris R-47 (TVAII), in order to improve efficiency of starch utilization. When phenyl-β-D-glucopyranoside was employed as an acceptor, β-D-glucopyranosides having maltooligosaccharide moiety at the non-reducing ends (degree of polymerization = 2-6) were obtained in good yields. This method employing ultrasound-irradiated substrate mixture and TVAII would be a promising tool for the direct conversion of starch into glycolipid.

Original language	English
Number of pages	1
Publication status	Published - 2006 Oct 19
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: 2006 May 24 → 2006 May 26

Other

Other	55th SPSJ Annual Meeting
Country	Japan
City	Nagoya
Period	06/5/24 → 06/5/26

Keywords

Effect
Irradiation/amylose/proximity
R-47
Thermoactinomyces
Vulgaris
α-amylase/transglycosylation/ ultrasound

ASJC Scopus subject areas

Engineering(all)

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Cite this

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title = "Direct conversion of starch into glycolipid by using Thermoactinomyces vulgaris R-47 α-amylase",

abstract = "We have reported that irradiation of ultrasound onto substrate mixture containing starch and glycolipid enhanced the transglycosylating activity of commercial α-amylase preparation. In this paper, we demonstrated a glycosyl-transferring reaction to various glycosyl acceptors employing α-amylase from Thermoactinomyces vulgaris R-47 (TVAII), in order to improve efficiency of starch utilization. When phenyl-β-D-glucopyranoside was employed as an acceptor, β-D-glucopyranosides having maltooligosaccharide moiety at the non-reducing ends (degree of polymerization = 2-6) were obtained in good yields. This method employing ultrasound-irradiated substrate mixture and TVAII would be a promising tool for the direct conversion of starch into glycolipid.",

keywords = "Effect, Irradiation/amylose/proximity, R-47, Thermoactinomyces, Vulgaris, α-amylase/transglycosylation/ ultrasound",

author = "Kenji Yamamoto and Atsushi Kobayashi and Hiroshi Ito and Takashi Tonozuka and Yoshiyuki Sakano and Shoda, {Shin Ichiro}",

year = "2006",

month = oct,

day = "19",

language = "English",

note = "55th SPSJ Annual Meeting ; Conference date: 24-05-2006 Through 26-05-2006",

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TY - CONF

T1 - Direct conversion of starch into glycolipid by using Thermoactinomyces vulgaris R-47 α-amylase

AU - Yamamoto, Kenji

AU - Kobayashi, Atsushi

AU - Ito, Hiroshi

AU - Tonozuka, Takashi

AU - Sakano, Yoshiyuki

AU - Shoda, Shin Ichiro

PY - 2006/10/19

Y1 - 2006/10/19

N2 - We have reported that irradiation of ultrasound onto substrate mixture containing starch and glycolipid enhanced the transglycosylating activity of commercial α-amylase preparation. In this paper, we demonstrated a glycosyl-transferring reaction to various glycosyl acceptors employing α-amylase from Thermoactinomyces vulgaris R-47 (TVAII), in order to improve efficiency of starch utilization. When phenyl-β-D-glucopyranoside was employed as an acceptor, β-D-glucopyranosides having maltooligosaccharide moiety at the non-reducing ends (degree of polymerization = 2-6) were obtained in good yields. This method employing ultrasound-irradiated substrate mixture and TVAII would be a promising tool for the direct conversion of starch into glycolipid.

AB - We have reported that irradiation of ultrasound onto substrate mixture containing starch and glycolipid enhanced the transglycosylating activity of commercial α-amylase preparation. In this paper, we demonstrated a glycosyl-transferring reaction to various glycosyl acceptors employing α-amylase from Thermoactinomyces vulgaris R-47 (TVAII), in order to improve efficiency of starch utilization. When phenyl-β-D-glucopyranoside was employed as an acceptor, β-D-glucopyranosides having maltooligosaccharide moiety at the non-reducing ends (degree of polymerization = 2-6) were obtained in good yields. This method employing ultrasound-irradiated substrate mixture and TVAII would be a promising tool for the direct conversion of starch into glycolipid.

KW - Effect

KW - Irradiation/amylose/proximity

KW - R-47

KW - Thermoactinomyces

KW - Vulgaris

KW - α-amylase/transglycosylation/ ultrasound

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T2 - 55th SPSJ Annual Meeting

Y2 - 24 May 2006 through 26 May 2006

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