Abstract
We have reported that irradiation of ultrasound onto substrate mixture containing starch and glycolipid enhanced the transglycosylating activity of commercial α-amylase preparation. In this paper, we demonstrated a glycosyl-transferring reaction to various glycosyl acceptors employing α-amylase from Thermoactinomyces vulgaris R-47 (TVAII), in order to improve efficiency of starch utilization. When phenyl-β-D-glucopyranoside was employed as an acceptor, β-D-glucopyranosides having maltooligosaccharide moiety at the non-reducing ends (degree of polymerization = 2-6) were obtained in good yields. This method employing ultrasound-irradiated substrate mixture and TVAII would be a promising tool for the direct conversion of starch into glycolipid.
Original language | English |
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Number of pages | 1 |
Publication status | Published - 2006 Oct 19 |
Event | 55th SPSJ Annual Meeting - Nagoya, Japan Duration: 2006 May 24 → 2006 May 26 |
Other
Other | 55th SPSJ Annual Meeting |
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Country/Territory | Japan |
City | Nagoya |
Period | 06/5/24 → 06/5/26 |
Keywords
- Effect
- Irradiation/amylose/proximity
- R-47
- Thermoactinomyces
- Vulgaris
- α-amylase/transglycosylation/ ultrasound
ASJC Scopus subject areas
- Engineering(all)