Direct conversion of starch into glycolipid by using Thermoactinomyces vulgaris R-47 α-amylase

Kenji Yamamoto, Atsushi Kobayashi, Hiroshi Ito, Takashi Tonozuka, Yoshiyuki Sakano, Shin Ichiro Shoda

Research output: Contribution to conferencePaperpeer-review

Abstract

We have reported that irradiation of ultrasound onto substrate mixture containing starch and glycolipid enhanced the transglycosylating activity of commercial α-amylase preparation. In this paper, we demonstrated a glycosyl-transferring reaction to various glycosyl acceptors employing α-amylase from Thermoactinomyces vulgaris R-47 (TVAII), in order to improve efficiency of starch utilization. When phenyl-β-D-glucopyranoside was employed as an acceptor, β-D-glucopyranosides having maltooligosaccharide moiety at the non-reducing ends (degree of polymerization = 2-6) were obtained in good yields. This method employing ultrasound-irradiated substrate mixture and TVAII would be a promising tool for the direct conversion of starch into glycolipid.

Original languageEnglish
Number of pages1
Publication statusPublished - 2006 Oct 19
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 2006 May 242006 May 26

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period06/5/2406/5/26

Keywords

  • Effect
  • Irradiation/amylose/proximity
  • R-47
  • Thermoactinomyces
  • Vulgaris
  • α-amylase/transglycosylation/ ultrasound

ASJC Scopus subject areas

  • Engineering(all)

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