Direct cloning of the brassica S locus by using a PI-derived artificial chromosome (PAC) vector

Go Suzuki, Masao Watanabe, Kinya Toriyama, Akira Isogai, Kokichi Hinata

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Self-incompatibility of Brassica is regulated by the S locus, which contains several genes including SLG and SRK. We found that both SLG and SRK genes were located at an approx. 80-kb MluI fragment in an S9 haplotype of B. campestris. Therefore, we cloned this MluI fragment into a BssHII site of the P1-derived artificial chromosome (PAC) vector. The utility of the direct cloning method is discussed in this study.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalGene
Volume199
Issue number1-2
DOIs
Publication statusPublished - 1997 Oct 15

Keywords

  • Brassica campestris
  • Complex genomes
  • S-locus glycoprotein
  • S-receptor kinase

ASJC Scopus subject areas

  • Genetics

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