@article{dddd620109734e758e21cf5ba5a1e037,
title = "Direct cloning of the brassica S locus by using a PI-derived artificial chromosome (PAC) vector",
abstract = "Self-incompatibility of Brassica is regulated by the S locus, which contains several genes including SLG and SRK. We found that both SLG and SRK genes were located at an approx. 80-kb MluI fragment in an S9 haplotype of B. campestris. Therefore, we cloned this MluI fragment into a BssHII site of the P1-derived artificial chromosome (PAC) vector. The utility of the direct cloning method is discussed in this study.",
keywords = "Brassica campestris, Complex genomes, S-locus glycoprotein, S-receptor kinase",
author = "Go Suzuki and Masao Watanabe and Kinya Toriyama and Akira Isogai and Kokichi Hinata",
note = "Funding Information: This work was supported in part by Grants-in-Aid for Special Research on Priority Areas (nos 07281102 and 07281103; Genetic Dissection of Sexual Differentiation and Pollination Process in Higher Plants) from the Ministry of Education, Science, Culture and Sports, Japan. The authors are grateful to Dr Pieter J. de Jong for providing us with the pCYPAC2 vector. The authors also wish to thank Drs Kunisuke Tanaka, Takehiro Masumura and Hideshi Makino, Kyoto Prefectural University, for their suggestions about techniques of megabase DNA isolation and gel extraction. G.S. is the recipient of a Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists.",
year = "1997",
month = oct,
day = "15",
doi = "10.1016/S0378-1119(97)00358-2",
language = "English",
volume = "199",
pages = "133--137",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",
}