Direct association of YY-1 with c-Myc and the E-box binding protein in regulation of glycophorin gene expression

Ji He Zhao, Toshiaki Inoue, Wataru Shoji, Yasuhisa Nemoto, Masuo Obinata

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We previously reported that YY-1, a versatile transcription factor, regulates expression of glycophorin gene by binding to its locus control region-like region (Gp-LCR) in combination with E-box binding protein during murine erythroleukemia (MEL) cell differentiation. In the present work, we demonstrated that YY-1 and c-Myc, a nuclear oncoprotein, were physically associated in vivo and that down regulation of c-Myc liberated free YY-1 from its complex, resulting in the functional binding of YY-1 to the Gp-LCR. We also showed that the E-box binding protein (EBP) which bound to E-box was physically associated with YY-1, facilitated binding of YY-1 to the neighboring site and their combinatorial binding may stimulate the GpLCR mediated enhancement of erythroid-specific transcription of glycophorin gene in MEL cells.

Original languageEnglish
Pages (from-to)1009-1017
Number of pages9
JournalOncogene
Volume17
Issue number8
DOIs
Publication statusPublished - 1998 Aug 27

Keywords

  • E-box binding protein
  • Glycophorin
  • Murine erythroleukemia cells
  • YY-1
  • c-Myc

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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