Direct association of LIS1, the lissencephaly gene product, with a mammalian homologue of a fungal nuclear distribution protein, rNUDE

Mayumi Kitagawa, Makiko Umezu, Junken Aoki, Hiroyuki Koizumi, Hiroyuki Arai, Keizo Inoue

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

LIS1 is a product of the causative gene for type I lissencephaly characterized by a smooth brain surface due to a defect in neuronal migration during brain development and a regulatory subunit of platelet-activating factor acetylhydrolase (PAF-AH). It is also a mammalian homologue of the fungal nuclear distribution (nud) gene, nudF, which controls the migration of fungal nuclei. Using the two-hybrid system, we identified a novel LIS1- interacting protein, rat NUDE (rNUDE), and found that it is a mammalian homologue of another fungal nud gene product, NUDE, and Xenopus mitotic phosphoprotein 43 which is phosphorylated in a cell cycle-dependent manner. rNUDE and the catalytic subunits of PAF-AH interact with the N- and C-termini of LIS1, respectively. However, these proteins, instead of simultaneously binding to LIS1, appeared to bind to LIS1 in a competitive manner. These results suggest that LIS1 functions in nuclear migration by interacting with multiple intracellular proteins in mammals. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalFEBS Letters
Volume479
Issue number1-2
DOIs
Publication statusPublished - 2000 Aug 11

Keywords

  • LIS1
  • NUDE
  • NUDF
  • Platelet-activating factor
  • Platelet-activating factor acetylhydrolase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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