Direct analysis of lipids in single zooplankter individuals by matrix-assisted laser desorption/ionization mass spectrometry

Yasuyuki Ishida, Oh Nakanishi, Shingo Hirao, Shin Tsuge, Jotaro Urabe, Tatsuki Sekino, Masami Nakanishi, Takashi Kimoto, Hajime Ohtani

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

A highly sensitive method to analyze the intact lipids in a single zooplankter individual at the level of a few tenths of a microgram was developed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with a direct sampling technique. The sampling procedure involved (1) putting a zooplankter individual sample onto the MALDI sample plate, (2) cutting the sample into a few pieces by means of tweezers, (3) depositing aliquots of matrix and cationization reagent solutions on the zooplankter sample, and (4) irradiating with a N2 laser to cause MALDI. By using this technique, the mass spectra of the single zooplankter samples showed a series of ions generated from phospholipids with 34 or 36 carbons in the acyl groups and neutral lipids such as triglycerides and diacylglyceryl ethers with 50-54 carbons in their acyl and alkenyl groups. Accordingly, this method enabled us to estimate the relative quantity between "structured lipids" (phospholipids) and "storage lipids" (neutral lipids) in an individual zooplankter, which should give us a good clue to elucidate the roles of each class of lipids in its growth.

Original languageEnglish
Pages (from-to)4514-4518
Number of pages5
JournalAnalytical Chemistry
Volume75
Issue number17
DOIs
Publication statusPublished - 2003 Sep 1
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Direct analysis of lipids in single zooplankter individuals by matrix-assisted laser desorption/ionization mass spectrometry'. Together they form a unique fingerprint.

Cite this