Diploid somatic-hybrid plants regenerated from rice cultivars

Kinya Toriyama, K. Hinata

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Somatic hybrid plants were obtained between rice cultivars 'Yamahoushi' and 'Murasakidaikoku'. Since 'Murasakidaikoku' is a double mutant having both dominant (purple coloration) and recessive (dwarf) markers, the somatic hybrids can be easily distinguished from their parents. Protoplasts were isolated from anther-derived calli, and electrofused protoplasts were cultured without selection of hybrid cells. Out of 27 regenerated plants, 9 proved to be hybrids based on their purple coloration and normal plant type, traits which were identical to those of the sexual F1 hybrid between the same parental cultivars. The somatic hybrids included three diploid and six triploid plants. Segregation of parental markers was observed in the selfed progenies. These results demonstrated that diploid hybrids of rice could be obtained through somatic hybridization between haploid anther-derived cells instead of by sexual hybridization.

Original languageEnglish
Pages (from-to)665-668
Number of pages4
JournalTheoretical and Applied Genetics
Volume76
Issue number5
DOIs
Publication statusPublished - 1988 Nov 1

Keywords

  • Anther culture
  • Protoplast fusion
  • Rice (Oryza sativa L.)
  • Somatic hybrid

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Fingerprint Dive into the research topics of 'Diploid somatic-hybrid plants regenerated from rice cultivars'. Together they form a unique fingerprint.

  • Cite this