Diffraction-unlimited optical imaging of unstained living cells in liquid by electron beam scanning of luminescent environmental cells

Hideki T. Miyazaki, Takeshi Kasaya, Taro Takemura, Nobutaka Hanagata, Takeshi Yasuda, Hiroshi Miyazaki

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

An environmental cell with a 50-nm-thick cathodoluminescent window was attached to a scanning electron microscope, and diffraction-unlimited near-field optical imaging of unstained living human lung epithelial cells in liquid was demonstrated. Electrons with energies as low as 0.8 - 1.2 kV are sufficiently blocked by the window without damaging the specimens, and form a sub-wavelength-sized illumination light source. A super-resolved optical image of the specimen adhered to the opposite window surface was acquired by a photomultiplier tube placed below. The cells after the observation were proved to stay alive. The image was formed by enhanced dipole radiation or energy transfer, and features as small as 62 nm were resolved.

Original languageEnglish
Pages (from-to)28198-28218
Number of pages21
JournalOptics Express
Volume21
Issue number23
DOIs
Publication statusPublished - 2013 Nov 18

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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