Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length polymorphism

Takuichi Sato, Junko Matsuyama, Nobuhiro Takahashi, Michiko Sato, Jolene Johnson, Charles Schachtele, Etsuro Hoshino

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

16S rDNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to generate restriction profiles of the reference strains, including the American Type Culture Collection type strains, of oral Actinomyces spp., i.e., A. israelii, A. gerencseriae, A. naeslundii genospecies 1 and 2, A. odontolyticus, A. meyeri and A. georgiae, and 23 Actinomyces strains isolated from human dental plaque. The 16S rRNA gene sequences from isolated genomic DNA samples were amplified by PCR. The PCR products were purified and characterized by single digestion with four restriction endonucleases, i.e., MnlI, HaeIII, CfoI, or HpaII. Among them, Mn/I was found to discriminate the respective reference strains. The clinical isolates were assigned to one of the species, i.e., A. gerencseriae, A. naeslundii genospecies 1 and 2 and A. odontolyticus, on the basis of their restriction profiles by single digestion with MnlI. Thus, 16S rDNA PCR-RFLP, using MnlI, is a rapid and reliable method for the differentiation of oral Actinomyces spp.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalArchives of Oral Biology
Volume43
Issue number3
DOIs
Publication statusPublished - 1998 Mar

Keywords

  • 16S rDNA
  • Actinomyces
  • Oral
  • PCR-RFLP

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

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