Differentiation of eosinophilic leukemia EoL-1 cells into eosinophils induced by histone deacetylase inhibitors

Kenji Ishihara, Aki Takahashi, Motoko Kaneko, Hiroki Sugeno, Noriyasu Hirasawa, Jang Ja Hong, Ok Pyo Zee, Kazuo Ohuchi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

EoL-1 cells differentiate into eosinophils in the presence of n-butyrate, but the mechanism has remained to be elucidated. Because n-butyrate can inhibit histone deacetylases, we hypothesized that the inhibition of histone deacetylases induces the differentiation of EoL-1 cells into eosinophils. In this study, using n-butyrate and two other histone deacetylase inhibitors, apicidin and trichostatin A, we have analyzed the relationship between the inhibition of histone deacetylases and the differentiation into eosinophils in EoL-1 cells. It was demonstrated that apicidin and n-butyrate induced a continuous acetylation of histones H4 and H3, inhibited the proliferation of EoL-1 cells without attenuating the level of FIP1L1-PDGFRA mRNA, and induced the expression of markers for mature eosinophils such as integrin β7, CCR1, and CCR3 on EoL-1 cells, while trichostatin A evoked a transient acetylation of histones and induced no differentiation into eosinophils. These findings suggest that the continuous inhibition of histone deacetylases in EoL-1 cells induces the differentiation into mature eosinophils.

Original languageEnglish
Pages (from-to)1213-1220
Number of pages8
JournalLife Sciences
Volume80
Issue number13
DOIs
Publication statusPublished - 2007 Mar 6

Keywords

  • Differentiation
  • EoL-1 cells
  • Eosinophils
  • Histone deacetylase inhibitor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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