Differentiation of a histiocytic lymphoma cell line by lipomodulin, a phospholipase inhibitory protein

Toshio Hattori, Thomas Hoffman, Fusao Hirata

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

When U 937 cells, a human histiocytic lymphoma cell line, were cultured with purified lipomodulin for 3 days, morphological and functional differentiation was induced as detected by microscopical examination of Giemsa stained smears, expression of mature monocyte antigen, and antibody dependent cellular cytotoxicity tests. Essentially similar differentiation was observed by the treatment with dexamethasone for 6 days and this differentiation by dexamethasone was blocked by monoclonal anti-lipomodulin antibody. Furthermore, the synthesis of immunoprecipitable lipomodulin in these cells was induced by dexamethasone treatment. These results, taken together, suggest that the induction of lipomodulin synthesis might be the primary event in dexamethasone-induced cellular differentiation of U 937 cells.

Original languageEnglish
Pages (from-to)551-559
Number of pages9
JournalBiochemical and biophysical research communications
Volume111
Issue number2
DOIs
Publication statusPublished - 1983 Mar 16

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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