TY - JOUR
T1 - Differentiation-dependent enhanced expression of protein phosphatase 2Cβ in germ cells of mouse seminiferous tubules
AU - Kato, Shunsuke
AU - Kobayashi, Takayasu
AU - Kusuda, Kazuyuki
AU - Nishina, Yukio
AU - Nishimune, Yoshitake
AU - Yomogida, Kentaro
AU - Yamamoto, Masayuki
AU - Sakagami, Hiroyuki
AU - Kondo, Hisatake
AU - Ohnishi, Motoko
AU - Chida, Naoki
AU - Yanagawa, Yuchio
AU - Tamura, Shinri
N1 - Funding Information:
Acknowledgements." We are grateful to K. Konno and F. Hayasaka for their technical assistance during the course of this study. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Culture and Sports of Japan, Uehara Memorial Foundation and Chiba-Geigy Foundation (Japan) for the Promotion of Science.
PY - 1996/11/4
Y1 - 1996/11/4
N2 - The presence of five distinct isoforms of protein phosphatase 2Cβ (PP2Cβ-1 ~ -5) is known. In this study, we demonstrate that the mRNA levels of PP2Cβ-3, -4 and -5 and PP2Cβ protein level increased during the course of the first wave of spermatogenesis in neonatal mouse testis. Northern blot and in situ hybridization analyses revealed that PP2Cβ-3, -4 and -5 were expressed predominantly in pachytene spermatocytes and in more highly differentiated germ cells. The substrate specificity of PP2Cβ-4 determined with artificial substrates differed from those of PP2Cβ-3 and -5, suggesting that the difference in the structure of PP2Cβ-3, -4 and -5 reflect their unique physiological functions in testicular germ cells.
AB - The presence of five distinct isoforms of protein phosphatase 2Cβ (PP2Cβ-1 ~ -5) is known. In this study, we demonstrate that the mRNA levels of PP2Cβ-3, -4 and -5 and PP2Cβ protein level increased during the course of the first wave of spermatogenesis in neonatal mouse testis. Northern blot and in situ hybridization analyses revealed that PP2Cβ-3, -4 and -5 were expressed predominantly in pachytene spermatocytes and in more highly differentiated germ cells. The substrate specificity of PP2Cβ-4 determined with artificial substrates differed from those of PP2Cβ-3 and -5, suggesting that the difference in the structure of PP2Cβ-3, -4 and -5 reflect their unique physiological functions in testicular germ cells.
KW - Isoform
KW - Protein phosphatase 2C
KW - Spermatogenesis
KW - Testicular germ cell
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U2 - 10.1016/0014-5793(96)01119-2
DO - 10.1016/0014-5793(96)01119-2
M3 - Article
C2 - 8915006
AN - SCOPUS:0030569396
VL - 396
SP - 293
EP - 297
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -