Differentiation and monitoring of cells using a biochip for regenerative medicine (differentiation of bone marrow stromal cells and myoblasts)

Fumihito Arai, Osamu Suzuki, Tomoyuki Uchida, Akihiko Ichikawa, Toshio Fukuda, Takenobu Katagiri, Ryutaro Kamijo, Masanori Nakamura, Mamoru Numata, Naruaki Watanabe

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST 2 cells) and myoblasts (C 2 C 12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.

Original languageEnglish
Pages (from-to)3239-3245
Number of pages7
JournalNihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C
Volume71
Issue number11
Publication statusPublished - 2005 Nov 1
Externally publishedYes

Keywords

  • Biological Engineering
  • Medical Engineering
  • Micromachine
  • Muscle and Skelton
  • Regenerative Medicine

ASJC Scopus subject areas

  • Mechanics of Materials
  • Mechanical Engineering
  • Industrial and Manufacturing Engineering

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