Differentiation and monitoring of cells using a biochip for regenerative medicine. (Differentiation of bone marrow stromal cells and myoblasts)

Tomoyuki Uchida, Fumihito Arai, Osamu Suzuki, Akihiko Ichikawa, Toshio Fukuda, Takenobu Katagiri, Ryutaro Kamijo, Masanori Nakamura, Mamoru Numata, Naruaki Watanabe

Research output: Contribution to journalArticlepeer-review

Abstract

A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST2 cells) and myoblasts (C2C12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.

Original languageEnglish
Pages (from-to)852-858
Number of pages7
JournalJSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
Volume49
Issue number3
DOIs
Publication statusPublished - 2007 Mar 15

Keywords

  • Biological engineering
  • Medical engineering
  • Micromachine
  • Muscle and skeleton
  • Regenerative medicine

ASJC Scopus subject areas

  • Mechanical Engineering
  • Industrial and Manufacturing Engineering

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