Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components

Osamu Takeuchi, Katsuaki Hoshino, Taro Kawai, Hideki Sanjo, Haruhiko Takada, Tomohiko Ogawa, Kiyoshi Takeda, Shizuo Akira

Research output: Contribution to journalArticle

2610 Citations (Scopus)

Abstract

Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.

Original languageEnglish
Pages (from-to)443-451
Number of pages9
JournalImmunity
Volume11
Issue number4
DOIs
Publication statusPublished - 1999 Oct

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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    Takeuchi, O., Hoshino, K., Kawai, T., Sanjo, H., Takada, H., Ogawa, T., Takeda, K., & Akira, S. (1999). Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components. Immunity, 11(4), 443-451. https://doi.org/10.1016/S1074-7613(00)80119-3