Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components

Osamu Takeuchi; Katsuaki Hoshino; Taro Kawai; Hideki Sanjo; Haruhiko Takada; Tomohiko Ogawa; Kiyoshi Takeda; Shizuo Akira

doi:10.1016/S1074-7613(00)80119-3

Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components

Osamu Takeuchi, Katsuaki Hoshino, Taro Kawai, Hideki Sanjo, Haruhiko Takada, Tomohiko Ogawa, Kiyoshi Takeda, Shizuo Akira

Research output: Contribution to journal › Article › peer-review

2730 Citations (Scopus)

Abstract

Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.

Original language	English
Pages (from-to)	443-451
Number of pages	9
Journal	Immunity
Volume	11
Issue number	4
DOIs	https://doi.org/10.1016/S1074-7613(00)80119-3
Publication status	Published - 1999 Oct
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S1074-7613(00)80119-3

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@article{a64e3bdc89004daeaed3c77a7632296f,

title = "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components",

abstract = "Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.",

author = "Osamu Takeuchi and Katsuaki Hoshino and Taro Kawai and Hideki Sanjo and Haruhiko Takada and Tomohiko Ogawa and Kiyoshi Takeda and Shizuo Akira",

note = "Funding Information: We thank T. Aoki for secretarial assistance and E. Nakatani and A. Maekawa for technical assistance. This work was supported by grants from the Ministry of Education of Japan.",

year = "1999",

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doi = "10.1016/S1074-7613(00)80119-3",

language = "English",

volume = "11",

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journal = "Immunity",

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TY - JOUR

T1 - Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components

AU - Takeuchi, Osamu

AU - Hoshino, Katsuaki

AU - Kawai, Taro

AU - Sanjo, Hideki

AU - Takada, Haruhiko

AU - Ogawa, Tomohiko

AU - Takeda, Kiyoshi

AU - Akira, Shizuo

N1 - Funding Information: We thank T. Aoki for secretarial assistance and E. Nakatani and A. Maekawa for technical assistance. This work was supported by grants from the Ministry of Education of Japan.

PY - 1999/10

Y1 - 1999/10

N2 - Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.

AB - Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.

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DO - 10.1016/S1074-7613(00)80119-3

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JO - Immunity

JF - Immunity

SN - 1074-7613

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ER -

Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components

Abstract

ASJC Scopus subject areas

UN SDGs

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