TY - JOUR
T1 - Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components
AU - Takeuchi, Osamu
AU - Hoshino, Katsuaki
AU - Kawai, Taro
AU - Sanjo, Hideki
AU - Takada, Haruhiko
AU - Ogawa, Tomohiko
AU - Takeda, Kiyoshi
AU - Akira, Shizuo
N1 - Funding Information:
We thank T. Aoki for secretarial assistance and E. Nakatani and A. Maekawa for technical assistance. This work was supported by grants from the Ministry of Education of Japan.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1999/10
Y1 - 1999/10
N2 - Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.
AB - Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.
UR - http://www.scopus.com/inward/record.url?scp=0033213590&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033213590&partnerID=8YFLogxK
U2 - 10.1016/S1074-7613(00)80119-3
DO - 10.1016/S1074-7613(00)80119-3
M3 - Article
C2 - 10549626
AN - SCOPUS:0033213590
VL - 11
SP - 443
EP - 451
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 4
ER -
