Differential regulation of diacylglycerol kinase isozymes in cardiac hypertrophy

Hirokazu Yahagi, Morihiko Takeda, Yasuhide Asaumi, Kenji Okumura, Ryotaro Takahashi, Jun Takahashi, Jun Ohta, Hiroko Tada, Yutaka Minatoya, Masahito Sakuma, Jun Watanabe, Kaoru Goto, Kunio Shirato, Yutaka Kagaya

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

To examine the involvement of diacylglycerol kinase (DGK) and phosphatidic acid phosphatase (PAP) in pressure overloaded cardiac hypertrophy, rats were subjected to either ascending aortic banding for 3, 7, and 28 days or sham operation. In comparison with sham-operated rats, the left ventricular (LV) weight of the aortic-banded rats increased progressively. At 28 days after surgery, the expression of DGKε mRNA but not DGKζ or PAP2b mRNA in the LV myocardium significantly decreased in the aortic-banded rats compared with the sham-operated rats. DGKζ protein in the LV myocardium translocated from the particulate to the cytosolic compartment in the aortic-banded rats. Furthermore, the myocardial content of 1,2-diacylglycerol and PKCδ protein expression in the particulate fraction of the LV myocardium significantly increased in aortic-banded rats compared with sham-operated rats. These results suggest that DGKε and DGKζ play distinct roles in the development of pressure overloaded cardiac hypertrophy and that the two isozymes are differentially regulated.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalBiochemical and biophysical research communications
Volume332
Issue number1
DOIs
Publication statusPublished - 2005 Jun 24

Keywords

  • 1,2-Diacylglycerol
  • Cardiac hypertrophy
  • Diacylglycerol kinase
  • Intracellular translocation
  • Phosphatidic acid phosphatase
  • Protein kinase C

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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