Differential protein profiles of Bradyrhizobium japonicum USDA110 bacteroid during soybean nodule development

Mika Nomura, Hatthaya Arunothayanan, Tan Van Dao, Hoa Thi Phuong Le, Takakazu Kaneko, Shusei Sato, Satoshi Tabata, Shigeyuki Tajima

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

During nodule development of legumes, together with the morphological alteration of plant cells, the rhizobia undergo marked biochemical and physiological changes and differentiate to bacteroids. Bacteroids invading a nodule are no longer free-growing cells, instead depending on the plant cell for all resources. To elucidate the molecular mechanism of the bacteroid differentiation of Bradyrhizobium japonicum, a time-course analysis of bacteroid protein profiles was studied by 2-D gel electrophoresis in soybean nodules. Using proteomic analysis, protein expressions in soybean nodule bacteroids 7, 10, 14, 28, and 49 days after inoculation (DAI) were monitored. The time points coincide with the early stage of nodule formation (7-10 DAI), the onset of nitrogen fixation (14-28 DAI), and nodule senescence (49 DAI). In this study, 275 annotated protein spots were successfully identified, and a cluster analysis of their expression was performed. A large portion of putative, upregulated proteins were observed in bacteroids at 7 or 10 DAI, and these upregulated proteins were mainly related to transcription, translation, protein folding, and degradation. In the later period (14-28 DAI) of bacteroid differentiation, a number of Nif and Fix proteins were upregulated. In addition, proteins related to the chaperonin and a synthetic enzyme of the poly-beta-hydroxybutyrate were expressed at high abundance. A number of proteins related to solute transporter were upregulated in the bacteroids and dominantly detected throughout nodule development. Changes of relative abundance of these proteins are discussed in relation to symbiosis.

Original languageEnglish
Pages (from-to)579-590
Number of pages12
JournalSoil Science and Plant Nutrition
Volume56
Issue number4
DOIs
Publication statusPublished - 2010 Aug
Externally publishedYes

Keywords

  • Bacteroid
  • Bradyrhizobium japonicum USDA110
  • Nodule
  • Proteomic analysis
  • Soybean

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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