Differential potencies of effector genes in adult Drosophila

Andreas S. Thum, Stephan Knapek, Jens Rister, Eva Dierichs-Schmitt, Martin Heisenberg, Hiromu Tanimoto

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The GAL4/UAS gene expression system in Drosophila has been crucial in revealing the behavioral significance of neural circuits. Transgene products that block neurotransmitter release and induce cell death have been proved to inhibit neural function powerfully. Here we compare the action of the five effector genes shibirets1, Tetanus toxin light chain (TNT), reaper, Diphtheria toxin A-chain (DTA), and inwardly rectifying potassium channel (Kir2.1) and show differences in their efficiency depending on the target cells and the timing of induction. Specifically, effectors blocking neuronal transmission or excitability led to adult-induced paralysis more efficiently than those causing cell ablation. We contrasted these differential potencies in adult to their actions during development. Furthermore, we induced TNT expression in the adult mushroom bodies. In contrast to the successful impairment in short-term olfactory memory by shibirets1, adult TNT expression in the same set of cells did not lead to any obvious impairment. Altogether, the efficiency of effector genes depends on properties of the targeted neurons. Thus, we conclude that the selection of the appropriate effector gene is critical for evaluating the function of neural circuits.

Original languageEnglish
Pages (from-to)194-203
Number of pages10
JournalJournal of Comparative Neurology
Volume498
Issue number2
DOIs
Publication statusPublished - 2006 Sep 10
Externally publishedYes

Keywords

  • Diphtheria toxin
  • GAL4/UAS system
  • Reaper
  • Tetanus toxin

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Thum, A. S., Knapek, S., Rister, J., Dierichs-Schmitt, E., Heisenberg, M., & Tanimoto, H. (2006). Differential potencies of effector genes in adult Drosophila. Journal of Comparative Neurology, 498(2), 194-203. https://doi.org/10.1002/cne.21022