Differential nociceptive responses in mice lacking the α1B subunit of N-type Ca2+ channels

Shinji Hatakeyama, Minoru Wakamori, Mitsuhiro Ino, Norimasa Miyamoto, Eiki Takahashi, Takashi Yoshinaga, Kohei Sawada, Keiji Imoto, Isao Tanaka, Toshihiro Yoshizawa, Yukio Nishizawa, Yasuo Mori, Tetsuhiro Niidome, Shin'ich Shoji

Research output: Contribution to journalArticlepeer-review

119 Citations (Scopus)

Abstract

The role of N-type Ca2+ channels in nociceptive transmission was examined in genetically engineered mice lacking the α1B subunit of N-type channels and in their heterozygote and wild-type littermates. In α1B-deficient mice, N-type channel activities in dorsal root ganglion neurons and spinal synaptoneurosomes were eliminated without compensation by other types of voltage-dependent Ca2+ channels. The α1B-deficient mice showed a diminution in the phase 2 nociceptive responses more extensively than in the phase I-nociceptive responses of the formalin test. The α1B-deficient mice exhibited significantly increased thermal nociceptive thresholds in the hot plate test, but failed to increase mechanical nociceptive thresholds in the tail pinch test. These results suggest a crucial role of N-type channels in nociceptive transmission, especially for persistent pain like phase 2 of the formalin test and for nociception induced by thermal stimuli.

Original languageEnglish
Pages (from-to)2423-2427
Number of pages5
JournalNeuroReport
Volume12
Issue number11
DOIs
Publication statusPublished - 2001 Aug 8
Externally publishedYes

Keywords

  • Antinociception
  • Dorsal root ganglion (DRG)
  • Knockout
  • Mouse
  • Pain

ASJC Scopus subject areas

  • Neuroscience(all)

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