Differential interference contrast microscope using photonic crystals for phase imaging and three-dimensional shape reconstruction

Laurent Fabre, Yoshihiko Inoue, Takafumi Aoki, Shojiro Kawakami

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We present a differential interference contrast microscope using photonic crystals capable of real-time capture of both phase and amplitude components independently without moving parts. Unlike previous methods using rotating polarizers to discriminate each component, we propose using a special camera equipped with an arrayed polarizer whose instant polarization measurement allows real-time acquisition of the phase gradient information. A two-image algorithm is used to reconstruct the phase twodimensional distribution of biological samples from the gradient information with a transmission-type microscope. We also talk about deducing a sample's three-dimensional shape for a reflection-type microscope. The efficiency of the method is demonstrated experimentally.

Original languageEnglish
Pages (from-to)1347-1357
Number of pages11
JournalApplied optics
Volume48
Issue number7
DOIs
Publication statusPublished - 2009 Mar 1

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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