Differential gene retention in plastids of common recent origin

Adrian Reyes-Prieto, Hwan Su Yoon, Ahmed Moustafa, Eun Chan Yang, Robert A. Andersen, Sung Min Boo, Takuro Nakayama, Ken Ichiro Ishida, Debashish Bhattacharya

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The cyanobacterium-derived plastids of algae and plants have supported the diversification of much of extant eukaryotic life. Inferences about early events in plastid evolution must rely on reconstructing events that occurred over a billion years ago. In contrast, the photosynthetic amoeba Paulinella chromatophora provides an exceptional model to study organelle evolution in a prokaryote-eukaryote (primary) endosymbiosis that occurred approximately 60 mya. Here we sequenced the plastid genome (0.977 Mb) from the recently described Paulinella FK01 and compared the sequence with the existing data from the sister taxon Paulinella M0880/a. Alignment of the two plastid genomes shows significant conservation of gene order and only a handful of minor gene rearrangements. Analysis of gene content reveals 66 differential gene losses that appear to be outright gene deletions rather than endosymbiotic gene transfers to the host nuclear genome. Phylogenomic analysis validates the plastid ancestor as a member of the Synechococcus-Prochlorococcus group, and the cyanobacterial provenance of all plastid genes suggests that these organelles were not targets of interphylum gene transfers after endosymbiosis. Inspection of 681 DNA alignments of protein-encoding genes shows that the vast majority have dN/dS ratios <<1, providing evidence for purifying selection. Our study demonstrates that plastid genomes in sister taxa are strongly constrained by selection but follow distinct trajectories during the earlier phases of organelle evolution.

Original languageEnglish
Pages (from-to)1530-1537
Number of pages8
JournalMolecular biology and evolution
Volume27
Issue number7
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Endosymbiosis
  • Paulinella
  • Photosynthesis
  • Primary plastids

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

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  • Cite this

    Reyes-Prieto, A., Yoon, H. S., Moustafa, A., Yang, E. C., Andersen, R. A., Boo, S. M., Nakayama, T., Ishida, K. I., & Bhattacharya, D. (2010). Differential gene retention in plastids of common recent origin. Molecular biology and evolution, 27(7), 1530-1537. https://doi.org/10.1093/molbev/msq032