Differential gene expression profiling between wild-type and ALAS2-null erythroblasts: Identification of novel heme-regulated genes

Tohru Fujiwara, Hideo Harigae, Shinichiro Takahashi, Kazumichi Furuyama, Osamu Nakajima, Jiying Sun, Kazuhiko Igarashi, Masayuki Yamamoto, Shigeru Sassa, Mitsuo Kaku, Takeshi Sasaki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To identify erythroid-specific heme-regulated genes, we performed differential expression analysis between wild-type and heme-deficient erythroblasts, which had been prepared from wild-type and erythroid-specific δ-aminolevulinate synthase-null mouse ES cells, respectively. Among 8737 clones on cDNA array, 40 cDNA clones, including 34 unknown ESTs, were first selected by their high expression profiles in wild-type erythroblasts, and evaluated further for their erythroid-lineage specificity, expression in hematopoietic tissues in vivo, and heme-dependent expression, which yielded 11, 4, and 4 genes, respectively. Because of the selection strategy employed, the final 4 were considered as the newly identified erythroid-specific heme-regulated genes. These 4 genes were uncoupling protein 2, nucleolar spindle-associated protein, cellular nucleic acid-binding protein, and a novel acetyltransferase-like protein. These findings thus suggest that heme may regulate a wide variety of hitherto unrecognized genes, and further analysis of these genes may clarify their role in erythroid cell differentiation.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalBiochemical and biophysical research communications
Volume340
Issue number1
DOIs
Publication statusPublished - 2006 Feb 3

Keywords

  • ALAS2
  • Erythropoiesis
  • Gene
  • Heme
  • cDNA array

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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