Differential gene expression of fibroblast growth factor receptor isoforms in rat ovary

Rei Asakai, Si young Song, Nobuyuki Itoh, Tohru Yamakuni, Kazuhiro Tamura, Ryohei Okamoto

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The gene expression of four fibroblast growth factor receptors (flg, bek, FGFR-3, and FGFR-4) in rat ovary cells was studied. Northern blot hybridization revealed that fig and bek mRNAs were detectable during all stages except a diestrus stage, whereas FGFR- 3 and FGFR-4 mRNAs were almost undetectable throughout the cycles. In situ hybridization also demonstrated that only flg and bek gene expression was detectable. A modest flg mRNA signal was detected in developing antral follicles and it was more prominent in the theca-interstitial cells than in the granulosa cells. A modest to weak flg mRNA signal was seen in the hypertrophied theca-interstitial cells of atretic follicles and a very weak flg mRNA signal was observed in the corpora lutea. On the other hand, a weak bek mRNA signal was seen in granulosa and theca-interstitial cells in developing follicles and also hypertrophied theca-interstitial cells of atretic follicles, but not in the corpora lutea. Intense signals for both fig and bek mRNAs were unexpectedly found in the epithelium of paroophoron at the hilus. These results demonstrate that the bFGF receptor isoforms are expressed differentially in the rat ovary cells.

Original languageEnglish
Pages (from-to)75-80
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume104
Issue number1
DOIs
Publication statusPublished - 1994 Aug
Externally publishedYes

Keywords

  • In situ hybridization
  • Rat ovary
  • bFGF receptor isoform

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Fingerprint

Dive into the research topics of 'Differential gene expression of fibroblast growth factor receptor isoforms in rat ovary'. Together they form a unique fingerprint.

Cite this