Differential expression pattern of C4 bundle sheath expression genes in rice, a C3 plant

Mika Nomura, Tomonori Higuchi, Yuji Ishida, Shozo Ohta, Toshihiko Komari, Nobuyuki Imaizumi, Mitsue Miyao-Tokutomi, Makoto Matsuoka, Shigeyuki Tajima

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)


NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PCK) are specifically expressed in bundle sheath cells (BSCs) in NADP-ME-type and PCK-type C4 plants, respectively. Unlike the high activities of these enzymes in the green leaves of C4 plants, their low activities have been detected in the leaves of C3 plants. In order to elucidate the differences in the gene expression system between C3 and C 4 plants, we have produced chimeric constructs with the β-glucuronidase (GUS) reporter gene under the control of the maize NADP-Me (ZmMe) or Zoysia japonica Pck (ZjPck) promoter and introduced these constructs into rice. In leaves of transgenic rice, the ZmMe promoter directed GUS expression not only in mesophyll cells (MCs) but also in BSCs and vascular cells, whereas the ZjPck promoter directed GUS expression only in BSCs and vascular cells. Neither the ZjPck nor ZmMe promoters induced GUS expression due to light. In rice leaves, the endogenous NADP-Me (OsMe1) was expressed in MCs, BSCs and vascular cells, whereas the rice Pck (OsPck1) was expressed only in BSCs and vascular cells. Taken together, the results obtained from transgenic rice demonstrate that the expression pattern of ZmMe or ZjPck in transgenic rice was reflected by that of its counterpart gene in rice.

Original languageEnglish
Pages (from-to)754-761
Number of pages8
JournalPlant and Cell Physiology
Issue number5
Publication statusPublished - 2005 May
Externally publishedYes


  • C plant
  • NADP-malic enzyme
  • Phosphoenol-pyruvate carboxykinase
  • Promoter analysis
  • Rice

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology


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