TY - JOUR
T1 - Differential expression pattern of C4 bundle sheath expression genes in rice, a C3 plant
AU - Nomura, Mika
AU - Higuchi, Tomonori
AU - Ishida, Yuji
AU - Ohta, Shozo
AU - Komari, Toshihiko
AU - Imaizumi, Nobuyuki
AU - Miyao-Tokutomi, Mitsue
AU - Matsuoka, Makoto
AU - Tajima, Shigeyuki
N1 - Funding Information:
We thank Dr. Kenzo Nakamura at Nagoya University for providing the plasmid pBI-Hm, and Dr. Jenq-Yunn Sheen at Harvard University for C4MeZm1CAT. This work was supported by a Grant-in-Aid for Scientific Research (Grant No. 15780047) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2005/5
Y1 - 2005/5
N2 - NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PCK) are specifically expressed in bundle sheath cells (BSCs) in NADP-ME-type and PCK-type C4 plants, respectively. Unlike the high activities of these enzymes in the green leaves of C4 plants, their low activities have been detected in the leaves of C3 plants. In order to elucidate the differences in the gene expression system between C3 and C 4 plants, we have produced chimeric constructs with the β-glucuronidase (GUS) reporter gene under the control of the maize NADP-Me (ZmMe) or Zoysia japonica Pck (ZjPck) promoter and introduced these constructs into rice. In leaves of transgenic rice, the ZmMe promoter directed GUS expression not only in mesophyll cells (MCs) but also in BSCs and vascular cells, whereas the ZjPck promoter directed GUS expression only in BSCs and vascular cells. Neither the ZjPck nor ZmMe promoters induced GUS expression due to light. In rice leaves, the endogenous NADP-Me (OsMe1) was expressed in MCs, BSCs and vascular cells, whereas the rice Pck (OsPck1) was expressed only in BSCs and vascular cells. Taken together, the results obtained from transgenic rice demonstrate that the expression pattern of ZmMe or ZjPck in transgenic rice was reflected by that of its counterpart gene in rice.
AB - NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PCK) are specifically expressed in bundle sheath cells (BSCs) in NADP-ME-type and PCK-type C4 plants, respectively. Unlike the high activities of these enzymes in the green leaves of C4 plants, their low activities have been detected in the leaves of C3 plants. In order to elucidate the differences in the gene expression system between C3 and C 4 plants, we have produced chimeric constructs with the β-glucuronidase (GUS) reporter gene under the control of the maize NADP-Me (ZmMe) or Zoysia japonica Pck (ZjPck) promoter and introduced these constructs into rice. In leaves of transgenic rice, the ZmMe promoter directed GUS expression not only in mesophyll cells (MCs) but also in BSCs and vascular cells, whereas the ZjPck promoter directed GUS expression only in BSCs and vascular cells. Neither the ZjPck nor ZmMe promoters induced GUS expression due to light. In rice leaves, the endogenous NADP-Me (OsMe1) was expressed in MCs, BSCs and vascular cells, whereas the rice Pck (OsPck1) was expressed only in BSCs and vascular cells. Taken together, the results obtained from transgenic rice demonstrate that the expression pattern of ZmMe or ZjPck in transgenic rice was reflected by that of its counterpart gene in rice.
KW - C plant
KW - NADP-malic enzyme
KW - Phosphoenol-pyruvate carboxykinase
KW - Promoter analysis
KW - Rice
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U2 - 10.1093/pcp/pci078
DO - 10.1093/pcp/pci078
M3 - Article
C2 - 15753103
AN - SCOPUS:20444419804
VL - 46
SP - 754
EP - 761
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 5
ER -